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IJMRI | International Journal of Multidisciplinary Research and Innovation
Published: October 20, 2026 | Vol. 1 Issue 4 | ISSN: 3139-5902
Research Article

ASSESSMENT OF THE PHYTOCHEMICAL AND PHYSICOCHEMICAL ANALYSIS OF THE LEAVES AND STEMS OF Vitex doniana

Ubiji, Chioma Ernest1✉,
Eze, Sunday Onyekwere1,
Nlemeke, Nkechinyere Francisca1,
Ukaegbu, Samson2
1 Department of Pure and Industrial Chemistry, Abia State University, Uturu, Nigeria
2 Department of Animal and Environmental Biology, Abia State University, Uturu, Nigeria
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Abstract

In this Assessment, the Samples of Vitex doniana (black plum) leaves and stems were collected from the black plum tree located in Abia State University Uturu. The leaves were air-dried, ground and sieved to fine powder and analysed for phytochemical and physicochemical properties including the determination of antibacterial and anti-fungal properties. The phytochemical screening of the leaves and stem bark of Vitex doniana shows the presence of phenol, alkaloid Saponins, Flavonoids and Tannins while the quantitative analysis showed a higher value in the amount of tannin present in the extracts. Tannins are naturally occurring plant polyphenols. Their main characteristic is that they bind and precipitate proteins and they have a large influence on the nutritive value of many foods eaten by humans and feedstuff eaten by animals.
Keywords: Vitex doniana (Black plum), Phytochemical screening, Tannins, Antibacterial and antifungal properties, Physicochemical properties

References

  1. A.O.A.C (1984). Phenolics in cereals, fruits and vegetables: Occurrence, extraction and malysis. J. Pharm Biodem. Anal, 41, 1523- 1542.
  2. Arbonnier, M. (2004), Frees, shrubs and lianas of West African dry zones, CIRAD MARGRAF publishers, GMBII AJ. Wageningen, the Netherland. 3(6) 573
  3. Aiwonegbe, A. E., Jyasele. J. U. and Izevbuwa, N. O. 2018) Department of Chemistry, University of Benin, Benin City, Nigeria He Journal of olence 3(20). 45-50
  4. De Bruyne T. Pieters L., Deelstra H. Vlietinck A. (2018), Condensed vegetables tannins biodiversity in structure and biological activities. Biochemical System Ecology, Texax 27-445 59.
  5. Egbekun, M.K., Akowe, J. I. and I.de, R.J. (2016). Physico-chemical Properties and Sensory Properties of Formulated Syrup from Black-plum (Vitex doniana) Fruit 49 (4): 301-306.
  6. George, F., Zohar K., Harinder PS, Makkar. Klaus B. (2013). The biological action of saponins in animal systems: a review. British Journal of Nutrition, 88: 587-605.
  7. Gyamfi MA, Aniya Y. (2012). Antioxidant properties of Thonningiam A, isolated from the African medicinal herb Thonningia sanguine. Biochemical Pharmacology. NY 63, 1725-37.
  8. Harborne JB, Baxter H. (2009). The handbook of natural flavonoids. Biochemistry. Volume 1 and 2. Chichester UK: pp 256-260.
  9. Hasler CM, Blumberg JB. (2015). Symposium on Phytochemicals: Biochemistry and Physiology. Journal of Nutrition. 129: 756S-757S.
  10. Iwu MM (2013). Handbook of African medicinal plants. Chemical Rubber Company. Benin City. 2(12-15).
  11. J. M. and Hutchison, J. (2015). Useful Plants of West Tropical Africa. Crown Agent, London, 34, 64-70.
  12. Lacaille-Dubois MA, Wagner H. (2011). Bioactive saponins from plants: An update. In Studies in Natural Products Chemistry; Atta-Ur-Rahman, ed. Elsevier Science. Amsterdam: 21: 633-687.
  13. Ladeji O. and Okoye (2014). Anti-hepatotoxic properties of Vitex doniana bank extract. Pharm. Biol. USA, 34 (5): 355-358.
  14. Ladji, O. and Okoye, Z.S.C. (2014). Anti-hepatotoxic properties of Vitex doniana bank extract. Pharm. Biol. USA. 34(5): 355-358.
  15. Mandal SM, Chakraborty D, Dey S (2010). Phenolic acids act as signaling molecules in plant-microbe symbioses. Plant Signal Behavior. Boston 5: 359-368.
  16. Mohammad, A., Tejas V. S., Ravindra L. B. and Pooja R. H. (2025). Phytochemicals: A review of their role in human health and disease prevention. GSC Biological and Pharmaceutical Sciences, 32(01), 299-307. DOI: 10.30574/gscbps.2025.32.1.0290
  17. Molyneux R.J., Nash RJ, Asano N (2016). Alkaloids: Chemical and Biological Perspectives, Vol. 11. ed. Pergamon. Oxford: 305-309.
  18. Myrtle, T., Pieters, L., Deeislra, H., Vlietinck, A. (2018). Condensed vegetables tannins: biodiversity in structure and biological activities, Biochemical System Ecology. Texas, 27: 445-59.
  19. Nigeria Natural Medicine Development Agency (NNMDA) (2008). Medical plants of Nigeria: South-East Nigeria. Vol.1. Lisida consulting Lagos Nigeria. pp. 204.
  20. Okigbo, R. N. (2011). Mycoflora within Black-plum (Vitex doniana Sweet) Fruits. Fruits. 56: 85-92.
  21. Pearson (1976). Antibacterial assessment of whole stem bark of Vitex doniana against some enterobacteriaceae. Kenya. African Journal of Biotechnology. 5(10): 958-959.
  22. Pridham JB (2000). In: Phenolics in Plants in Health and Disease, Pergamon Press, New York: 34-35.
  23. Shofowora A. E. (2013). Medicinal plants and traditional medicine in Africa. Spectrum books ltd. Amsterdam: 2nd Edn. pp 26-100.
  24. Tadzabia, K., Maina, H.M., Manera, O.N. and Osunlaia, A.A. 2013. Elemental and physiochemical screening of Vitex doniana leaves and stem bark in Hong Local Govt.
  25. Matthia K. (2011). Nutrition in the Adult Years. In Krause’s Food, Nutrition, and Diet Therapy, 10th ed. L.K. Mahan and S. Escott-Stump; 271: 274-275.
  26. Wink, M., Schmeller, T., Latz-Bruning, B. (2018). Modes of action of allelochemical alkaloids: Interaction with neuroreceptors, DNA and other molecular targets. Journal of chemical Ecology. Boston: 24: 2018-1937.
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